Molecular Biology/Genetics USMLE Step 1 Practice Question
DNA, extracted from a patient, is to be tested by PCR and DNA sequencing to ascertain the presence of a particular mutation. The DNA sequence to be amplified by PCR is shown below. The dashed line indicates the internal sequence where the potential mutation is located. Which answer choice represents the pair of primers that should be used in the PCR? (Sequence: TCCTGAGC--------------AAATGTGT)
Answer choices
- AGCTCAGGA and AAATGTGT
- BTCCTGAGC and TTTACACA
- CAGGACTCG and TTTACACA
- DGCTCAGGA and ACACATTT
- ETCCTGAGC and ACACATTTCorrect answer
- FTCCTGAGC and TGTGTAAA
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